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Comment · Fri, June 13, 2025 · ND Owner

Anthocyanin-fucoidan nanocomplex

Original post in this thread

Warren_sl · 10 points

I thought this would be an interesting thing to share. Unsure if stacking Fucoidans and anthocyanins confer any similar benefit or not, but this could be an interesting product type to look into. Curious on its modulation of SIRT6 considering the affinity both Fucoidans and Anthocyanins have for it.

“Abstract

Anthocyanins, commonly extracted from aronia, exhibit excellent in antioxidant activity and anti-cancer activity. However, anthocyanins are not only easily oxidized in water but also rapidly disappear from the body, thus requiring a large amount of administration. To solve these limitations, we selected fucoidan, an anionic polymer, to produce an anthocyanin-fucoidan nanocomplex (AFNC) with enhanced absorption and chemical stability by ionic bonding and π-π stacking between anthocyanins. In vitro, AFNC showed increased cell permeability absorption and plasma chemical stability than free anthocyanins. AFNC suppressed the epithelial mesenchymal transition (EMT) signal including IκBα/NF-κB signaling pathway and the release of pro-inflammatory cytokines. In vivo, AFNC exhibited 3.24-fold higher bioavailability than free anthocyanin in rats. AFNC effectively suppressed the gene…

What they were answering

Allonsy82 · 4 points

I used to use them, they are “supposed” to be a reputable company and are a popular brand in the anti ageing/longevity space

Appreciate your input into this and their company. Something of note that their CEO has said multiple times, including this interview here (at the beginning) is that apparently they test each batch to ensure sirt6 activation as some batches actually show no impact on sirt6 and some even inhibited it. Now I’m no expert in this area of science but surely this is marketing and perhaps flat out lying? There’s no evidence of this in any study and is that even possible in the first place? Just seems a bit sketchy

https://youtu.be/einqImMpHJc?si=bQ8QJm9WtwDxxkXu

u/MisterYouAreSoDumb · ND Owner

Right, and it has pissed me off for a long time... if you can't tell. They are "supposed" to be a reputable company, but all I can find on that is that they just say they are, and people repeat it. Where it the evidence? Where are the lab testing reports and COAs? Where are the supposed clinical studies run with their products that they keep repeating over and over? Just link us the peer-reviewed papers. Where is the evidence of all their profits going into funding research? If it was all true, that would be wonderful! I want more people doing what we are doing. I don't want to be the only one trying to push the science forward. It makes me a pissed of jaded individual having to constantly be shown that I am. If other people would jump on that train with me, I would applaud it! Hell, I would even partner with them to help join forces on things. My team and I are actually doing what they claim to, but we do it quietly in the background. We don't blast it on every page of our site like we are trying to beat customers over the head with it. And honestly, if they were actually doing it and showing evidence, I would be okay with them beating everyone over the head with it like they are on the site. If you are doing the work, you get to pat yourself on the back if you want to. That's fine. My issue is that literally every single time I go down this route in this industry, and look into a brand making claims, they ALWAYS turn out to be lies. I have been buying products on the supplement market, and testing them in analytical labs for almost 14 years now. Today is the 12th anniversary of me founding this company, but I was doing the testing thing for the community before I ever even thought of starting a company. I have lived, breathed, and bled this for a long time. How many times do you think I have been pleasantly surprised by lab results we have gotten back from market testing of other brands we have done, or after I have looked into claims other brands were making about their efforts? I can count the times on one hand, and you should see the retained sample section of our facility. We have thousands of bottles of stuff from brands over the years.

Our efforts to push the standards of this industry forward don't just focus on brands, either. I have found so many things wrong with every part of this shitty industry. Fake "dry labs" doing no testing, and just giving passing COAs to brands that pay them. Real labs that are just doing shitty science. Massively large reputable labs doing questionable chemistry, or participating in actions that I feel are consumer fraud. Industry organizations in bed with the brands that pay them, rather than advocating for consumers. Organizations that claim to be consumer protection entities that are actually just marketing arms of the brands themselves. Education and review sites set up by the brands themselves to trick consumers and funnel them back to the brand's site to buy stuff. Contract research organizations doing studies for brands that falsify data, or bend the methodologies to show statistical significance that is not actually there. Scientists who are willingly putting their names on papers with false or misleading claims. Contract manufacturers that lie to brands and falsify master batch records. Suppliers that falsify extraction ratios and standardizations, or come up with sophisticated adulteration schemes to put out fake or impure products, but trick the available lab methods. You name it, and I have seen it. It's over and over and over again. The deeper I look, the more absolute fraud I find. It's just constant for 14 years now. In fact, today is the 12th anniversary of me founding this company. However, I was doing this testing and calling out bad actors long before I ever even thought of starting a company. It's just been so much shit I have found for so long... it really makes me jaded at everyone.

So this is why I take the stance I do a lot, and just don't believe anyone without evidence anymore. I have been let down too many times to give people the benefit of the doubt, and in my experience, the ones making claims the loudest are most often the ones doing none of what they claim. The people that are doing the real work tend to just focus on that work, and let the work speak for itself. They don't engage in puffery to talk themselves up constantly. If they do talk about something good they have done, they go into detail on it, and show evidence of that. They talk with passion about what they have accomplished, and usually set expectations for what people should take away from that more reasonably. The ones you see regularly and constantly referring to all this good stuff they are doing without showing evidence of it, and making up stupid terms to redefine themselves to seem like sometime greater than they are, are usually just lying to everyone. I've just seen it too many times now. Of course I would love to be proven wrong, and I will absolutely give credit where credit is due, if I am shown evidence of those efforts. That just rarely ever happens. Then when it does, I still seem to get shit on, like with the Greg Doucette thing.

The HTLT team absolutely was selling fake turkesterone the first time we tested, just like everyone else in the industry. However, they took our data and what we were saying, then went and did something with it. They actually went out and fixed the issue. I thought highlighting that would be a good thing, and that people would appreciate it, but more than anything it just made people shit on us for it. They are literally one of the only brands that have ever seen us post failing results on one of their products, and rather than just attacking me, they went out and improved their product. That's not me saying I approve of everything they do, nor is it me saying it was okay to sell fake product the first time out. However, if people don't ever get props for admitting they were doing wrong, then changing their behavior, what's the incentive for anyone to do it?

So do I know for a fact that Do Not Age is just lying to everyone about what they claim? No, I do not. However, I have been shown absolutely no evidence of any of their claims. Furthermore, the things they do show me set off all the red flags from my efforts in this industry for over a decade. I am more than willing to be wrong on them, but the evidence I have at this point doesn't point that way. If they have some, by all means show it.

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