Comment · Mon, February 21, 2022 · ND Owner
Slc12a8 possibly not an NMN transporter?
Original post in this thread
__shamir__ · 3 points
I came across an article, ["Absence of evidence that Slc12a8 encodes
a nicotinamide mononucleotide transporter"](https://sci-hub.hkvisa.net/10.1038/s42255-019-0085-0#), which appears to debunk [at least some of] the evidence presented in Slc12a8 is a nicotinamide mononucleotide transporter for Slc12a8's action as an NMN transporter. The article is quite brief so I won't quote from it, but it basically disputes the validity of the techniques used (especially from a chemistry / biochemistry perspective from what I can gather)
OTOH, some of the findings in Slc12a8 is a nicotinamide mononucleotide transporter seem pretty definitive. But if the argument of the critique, namely that the analytical methods were invalid/flawed holds, then I suppose it can't necessarily be trusted. Here's some quotes from a relevant subsection, "Biochemical features of the Slc12a8 protein":
> Next, we overexpressed the full-length mouse Slc12a8 cDNA in mouse NIH3T3 cells. We chose this cell line because it does not have any detectable extracellular activities of CD73 (converting NM…
What they were answering
chris106 · 2 points
Looking forward to the NMN powder - 1 coated tablet is actually already too much for me.
I suppose this might have been answered already, but can't find it right now -
How is the NMN made stable in powder form?
Wasn't it made into coated tablets because it degrades rather fast at room temperature, if not protected?
u/MisterYouAreSoDumb · ND Owner
The synthesis route was changed for NMN. The previous synthesis led to a final powder that was not stable at room temp. It would degrade quickly. We are not sure if that was a function of the crystal structure or some minor impurity left that was resulting in increased degradation. However, since the synthesis change we have seen no degradation like we were seeing before, so we are comfortable now will offering it in powder form. That's a good thing, as it was a pain in the ass to keep the raw NMN at freezing temps right up until the production process. Now we can store it sealed at room temperature without issue, which simplifies the storage and production process.