Comment · Mon, February 21, 2022 · ND Owner
Slc12a8 possibly not an NMN transporter?
Original post in this thread
__shamir__ · 3 points
I came across an article, ["Absence of evidence that Slc12a8 encodes
a nicotinamide mononucleotide transporter"](https://sci-hub.hkvisa.net/10.1038/s42255-019-0085-0#), which appears to debunk [at least some of] the evidence presented in Slc12a8 is a nicotinamide mononucleotide transporter for Slc12a8's action as an NMN transporter. The article is quite brief so I won't quote from it, but it basically disputes the validity of the techniques used (especially from a chemistry / biochemistry perspective from what I can gather)
OTOH, some of the findings in Slc12a8 is a nicotinamide mononucleotide transporter seem pretty definitive. But if the argument of the critique, namely that the analytical methods were invalid/flawed holds, then I suppose it can't necessarily be trusted. Here's some quotes from a relevant subsection, "Biochemical features of the Slc12a8 protein":
> Next, we overexpressed the full-length mouse Slc12a8 cDNA in mouse NIH3T3 cells. We chose this cell line because it does not have any detectable extracellular activities of CD73 (converting NM…
What they were answering
InformationIcy4989 · 1 points
FYI Brenner discovered that NR is a precursor to NAD.
How much weight should we put on conflicts of interest anyway, isn't the methodology what matters?
u/MisterYouAreSoDumb · ND Owner
Conflicts matter when their data makes no sense. There is a history of Chromadex putting out anti-NMN articles over the years, and them trying to assert patent control over nicotinamide riboside (NR). It's been a constant thing for years now, so the skepticism of Chromadex's intentions are warranted. Grozio is not putting out anti-NR data, or paying for anti-NR articles to be written online to sway public opinion, nor is Grozio trying to exert exclusive patent control over NMN. The motivations behind the two sides seem very different from one another, and her explanation in the reply paper makes sense to me and my scientific team. Their methodologies seem sound. The arguments against their paper on Slc12a8 don't match up, and the history of Chromadex's attempts to steer people away from NMN to NR make me question the scientific integrity of the information they are putting out.