Comment · Wed, February 23, 2022 · ND Owner
Slc12a8 possibly not an NMN transporter?
Original post in this thread
__shamir__ · 3 points
I came across an article, ["Absence of evidence that Slc12a8 encodes
a nicotinamide mononucleotide transporter"](https://sci-hub.hkvisa.net/10.1038/s42255-019-0085-0#), which appears to debunk [at least some of] the evidence presented in Slc12a8 is a nicotinamide mononucleotide transporter for Slc12a8's action as an NMN transporter. The article is quite brief so I won't quote from it, but it basically disputes the validity of the techniques used (especially from a chemistry / biochemistry perspective from what I can gather)
OTOH, some of the findings in Slc12a8 is a nicotinamide mononucleotide transporter seem pretty definitive. But if the argument of the critique, namely that the analytical methods were invalid/flawed holds, then I suppose it can't necessarily be trusted. Here's some quotes from a relevant subsection, "Biochemical features of the Slc12a8 protein":
> Next, we overexpressed the full-length mouse Slc12a8 cDNA in mouse NIH3T3 cells. We chose this cell line because it does not have any detectable extracellular activities of CD73 (converting NM…
What they were answering
chris106 · 2 points
As always- thanks for the detailed response!
Looking forward to giving lower doses a try,
and less cost is a welcome aspect, too!
u/MisterYouAreSoDumb · ND Owner
My pleasure! We have other NAD+ stuff coming out, too. So you might find a low dose of NMN paired with some other NAD+ supplements might do the trick for you.