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Comment · Sun, February 20, 2022 · ND Owner

Slc12a8 possibly not an NMN transporter?

Original post in this thread

__shamir__ · 3 points

I came across an article, ["Absence of evidence that Slc12a8 encodes
a nicotinamide mononucleotide transporter"](https://sci-hub.hkvisa.net/10.1038/s42255-019-0085-0#), which appears to debunk [at least some of] the evidence presented in Slc12a8 is a nicotinamide mononucleotide transporter for Slc12a8's action as an NMN transporter. The article is quite brief so I won't quote from it, but it basically disputes the validity of the techniques used (especially from a chemistry / biochemistry perspective from what I can gather)

OTOH, some of the findings in Slc12a8 is a nicotinamide mononucleotide transporter seem pretty definitive. But if the argument of the critique, namely that the analytical methods were invalid/flawed holds, then I suppose it can't necessarily be trusted. Here's some quotes from a relevant subsection, "Biochemical features of the Slc12a8 protein":
> Next, we overexpressed the full-length mouse Slc12a8 cDNA in mouse NIH3T3 cells. We chose this cell line because it does not have any detectable extracellular activities of CD73 (converting NM…

What they were answering

LungsOfSteel · 2 points

Yeah, registered by Seneque, which partners with the Buck Instute for Research of Aging. Seneque domain was registered in 2013. Elevant is featured out of 3 engineered by Seneque lab.

https://seneque.com/engineered-by-seneque/

That said… Having not tried neither NR nor NMN I am still only interested in testing NMN but it’s too expensive for me right now.

Until then I will continue to use niacin as my NAD+ booster and quercetin for the CD38 inhibition. I really enjoy the flush ¯\_(ツ)_/¯

u/MisterYouAreSoDumb · ND Owner

Interesting... I will do some more research. I am always skeptical of undisclosed affiliations.

I do think NR, niacin, and nicinamide have their place, too. That's especially true when combined with quercetin. Some people do like the niacin flush as well. We are going to be releasing more NAD+ supplements soon. This includes non-enteric NMN in capsules and powder for a lower price, along with other compounds that are in the NAD+ cycle. It is a complex cycle, and only supplementing for one part of that cycle isn't always the best strategy.

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