Comment · Fri, August 21, 2015 · Ceretropic
Anyone else interested in P21 nasal spray from Ceretropic?
Original post in this thread
SimianSound · 18 points
I'm hoping this post serves as a petition of sorts to the awesome vendors over at Ceretropic. Show your support if you're also waiting on this!
What they were answering
SangersSequence · 3 points
Lucigen just sells an endotoxin free e coli strain that is apparently good for protein expression, although that might be a good place to start. I don't really know anyone who does peptide biosynthesis commercially, just for research. So I don't really know who you could talk to about it.
A trick is going to be expressing the protein in such a way that you can purify and subsequently remove the purification tag (Poly-His usually), which means including a protease cleavage site which often leave residual amino acids behind. SUMO Protease, in the e coli system we used leaves two, TEV used in Daedalus leaves a single Glycine. Factor Xa clevage from the purification tag might be the best option as it doesn't have residual sequence. You'd still need to pass through HPLC for final purification before analysis, HIS purification is still kind of messy.
You can read more about e coli fusion protein production here: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242369/
The patent for the Daedalus system which has some relevant information: http://www.google.com/patents/WO2014093403A1?cl=en
I suppose the technology for this might not be mature to the point where it is cost effective for large scale, small peptide production vs solid phase (although it's how they make insulin) but the most expensive part, other than the growt…
u/MisterYouAreSoDumb · Ceretropic
Ohh, ok. So Lucigen is just selling the e coli strain to be used in the process. I am going to have to do a lot more reading on it. I'm sure it would take some work to get it to a point that it would be cost effective for our purposes.