Comment · Sat, May 21, 2016
Mk-677
Original post in this thread
metoo79 · 11 points
I was curious how testing was going on the latest batches. Thanks!
What they were answering
Direct reply to the original post — see the thread post above.
u/MisterYouAreSoDumb
Still impure. So far all 6 batches this year, from multiple suppliers, has been impure. It's a solvent residue with a carbonyl group, which is giving an extra peak at about 1,730-1,740 cm-1. HPLC shows the impurity is a few percent, with the total purity of the batches this year ranging from 92-96%. I looked back through the synthesis methods for MK-677, and the only solvent used in the synth that has a carbonyl group is benzyl chloroformate. That step gives yields of about 80%. If they do not purify it before moving onto the next step in the synth, the benzyl chloroformate could be left in, giving us the extra peak at ~1,740. When you FTIR Sigma Aldrich's reference standard, there is no peak at 1,740. Also, the less pure batches via HPLC are the ones that smell the worst, and coincide with larger peaks at 1,740. So the presence of that peak on the FTIR shows the impurity, and the HPLC and UPLC we ran confirms it ranges from 3-6%. If it is benzyl chloroformate, that is nasty stuff, and very toxic. I also just tested another vendor's MK-677 (Hardcore Formulations), and it has the 1,740 peak. So I would venture to guess it is the same impure shit we've been getting from the labs.