Comment · Wed, October 20, 2021 · ND Owner
Reddit AMA | In-House Lab Edition
Original post in this thread
NootropicsDepotGuru · 77 points
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We are very excited to announce that Nootropics Depot will be conducting its first-ever Reddit AMA (Ask Me Anything), which is a semi-live Q&A session, about our In-House Analytical Laboratory and we would love for you to join us! See below for full details and get your question(s) ready. We hope to see you there!
WHEN
STARTS: October 20th | 9am AZ MST
ENDS: October 22nd | 9am AZ M…
What they were answering
gene_n_tacos · 1 points
I was very impressed with the dry and wet lab equipment shown on the virtual laboratory. For small molecule nootropics like phenibut and aniracetam for example, you mentioned that you have done proton NMR. Where are these samples sent to do this analysis?
u/MisterYouAreSoDumb · ND Owner
That depends on how specific or advanced the methods are. I can't divulge the names of our partners, as we are under NDAs, but we have a few different labs we use. We have one partner for our normal basic NMR work. They like running samples through their machine and giving data, but they don't want to interpret it. So for that partner we use them for routine QC work, then analyze the data ourselves. This helps keep the cost down, as they only need to run samples and give data. They don't have to take up time for their expensive PhD scientists to interpret the data. Our lab director takes the raw data from their NMR and interprets it.
For more advanced work like two dimensional quantitative NMR, we use a different lab. They have more advanced NMR machines, and their analysts are much more advanced and qualified in the more esoteric NMR methods/methodologies. This is obviously much more expensive. I am talking $2,000 to $10,000 a sample in some cases. However, sometimes that is necessary for the specific molecule we are looking at. We have used them on peptides a lot, as they can use the 2D NMR to really identify the peptides in much more detail than other methodologies.
For most of our QC identification work, FTIR, LC-MS, HP-TLC, and UV-Vis are used. It just depends on the specific sample. Some samples have plenty of carbon signals, so IR is good. Some don't, so mass spec or NMR is needed. Polymorphs come into play a lot with IR, too. Some molecules act strange with IR because of their crystal structure. In those instances we use orthogonal techniques to ensure we ID properly. Sometimes that means using both FTIR, NMR, and mass spec data.