Comment · Thu, January 9, 2020
New bacopa pills, 24% bacosides
Original post in this thread
jhonny_derp · 8 points
Interesting new offering. But the big question is, does it make you tired or lethargic? Like Bacognize?
What they were answering
throwawayy913 · 3 points
"Congratulations, you have found the most potent bacopa we have ever tested! This Bacopa monnieri extract came in at a whopping 24% Bacosides by HPLC/UPLC! You may think to yourself: "Wait a second, I have seen Bacopa monnieri extracts with a Bacoside content of over 50%?" It is true that there are bacopa extracts out there claiming to be over 50% bacopasides. However, something that is not often discussed is that these testing values are most often obtained by UV-VIS, or ultraviolet-visual spectromic analysis. UV-VIS is an old methodology that not only measures the Bacosides, but all other compounds that have a UV signal remotely similar to bacosides. This is because it does not separate the compounds before it measures them. It groups them all together. This means that it grossly inflates testing values. As a manufacturer, listing a Bacoside content of over 50% is fantastic, as it will drive sales. However, the consumer is getting massively mislead by those numbers. This is why we test our own Bacopa monnieri extract strictly by the much more accurate HPLC technology. In fact, we test via even more advanced UPLC machinery, or ultra high performance liquid chromatography. Our in-house lab at Nootropics Depot has one of the most advanced UPLCs in the world from Waters, which runs at pressures up to 15,000 psi, which is over double the pressures run on a normal HPLC. This allow…
u/MisterYouAreSoDumb
We just got a second UPLC, too! This one has an automated column switcher and hooks into a mass spec detector. So now we will have not just a mass spec in house, but one that will be more accurate and flexible. This is because of the UPLC and PDA detector we have plumbed inline with it. Most LC-MS machines are standard LCs with only a mass spec detector. So you get limited chromatographic abilities and only mass data. Our UPLC-MS has an automated column switcher, quaternary pump, auto-sampler, then a full PDA detector in front of the mass detector. So we can have different columns that auto-switch in the machine. We can have FOUR different solvents mixing throughout our runs, which makes complex methods possible. Then we can see the full UV spectrum like we would on a high end HPLC/UPLC, PLUS then we get the mass data at the end. It's like the ultimate R&D and method development/validation setup! The only way we could make it more advanced is to hook it into a triple quad mass spec detector, but that was like another $250,000-$500,000. So I held off on that one for now. Eventually we will. I just spent a ton of money on lab equipment this year, so I need to make sure I am not going too nuts. LOL