Comment · Tue, June 14, 2022 · ND Owner
Lab Testing Results Of Turkesterone, Beta Ecdysterone, and Gorilla Mind Sigma
Original post in this thread
MisterYouAreSoDumb · 866 points
Okay, so I am finally getting around to make a post about the results of some of the lab testing we have been doing. I've had the results for a bit, but I've been holding off on officially releasing them because frankly it stresses me out these days. It's the same song and dance every time, and it is one I have been doing for a decade now. I release results showing other products are not what they claim, then people attack me and claim I am lying to sell more product. Sometimes the attacks are relatively harmless, and just raise my blood pressure a bit for a day or two. Other times they are death threats to me, my family, and my team. It's not fun sometimes. You'll notice I don't do this nearly as much as I did back in the day on /r/Nootropics. I was younger and more gung-ho then, and had a lot less on my plate. Plus, my family and team kind of pleaded with me to stop doing it so much because of all the attacks and threats. This is why I have not been releasing things like this as much these days. It's not because product quality magically got better in the industry, I can tell you that much! Anyway, let's just get into it.
I woke up this morning ready to jump right into this pos…
What they were answering
No_Neighborhood_5919 · 2 points
I think it would be beneficial for you to clarify a few points:
How were the samples prepared for analysis? I think it is essential to rule out any limitations of the analysis which could lead to low recoveries from the capsules. Including how much of the Gorilla Mind products were used for sample preparation. Were several samples pooled together to produce a representative sample or is each sample a reflection of one capsule?
Could you present all chromatograms, including each of those used for the calibration and samples? With this it would be good to clarify how you calculated your LOD/LOQ.
It does appear to me that it is clear in the chromatograms that turkesterone does not appear to be present in high quantities in the sample but I think further transparency is required.
u/MisterYouAreSoDumb · ND Owner
Okay, so it was a composite. The composite was made from 20 capsules. The average fill weight of the capsules (551.54mg) was calculated by emptying 10 capsules and weighing the contents.
Sample prep for prep 1 was mixing 133mg of sample in 50ml of methanol and sonicating for 30min at 40°C.
Sample prep 2 was mixing 155.9mg in 50ml of methanol and sonicating for 30min at 40°C.
We also always do validation injections for every failing results. Initial injections were run Friday 04/22/22 for both prep 1 and prep 2. Both results were OOS, or out of spec from label claim. When that happens, we automatically do new injections to confirm that we don't see drastic differences in results. If differences are seen, we run a full OOS investigation automatically. Injections 3 and 4 were run on Monday 04/25/22. That means samples of the Gorilla Mind turkesterone product had 4 separate UPLC runs that all agreed with each other.
LoD/LoQ is made using the USP <621> methodology using SNR of peak height compared to the noise floor.
Here is the calculation of concentration
Here is the chromatogram of the calibration using the Sigma Aldrich reference standard
Here is the chromatogram of Gorilla Mind prep 1 run on 04-25-22
Here is the chromatogram of Gorilla Mind prep 2 run on 04-25-22