Comment · Thu, June 9, 2016
status of 7,8-dihydroxyflavone ?
Original post in this thread
lorrieh · 1 points
I know that MrY mentioned a few months ago that his samples didn't match the Sigma-Aldrich reference samples, and he didn't feel comfortable selling this compound until he could explain the discrepancy.
so where are we on that?
What they were answering
ebowden · 0 points
Jesus, one might have to collect more knowledge by measuring the affinities of a whole bunch of other flavones. Sounds expensive. Would this be in any way useful as a complement of sorts? I imagined an extra hydroxyl group to be easy to detect, but I really know very little. Are there other dihydroxyflavones that bind TrkB? If there are, I doubt their affinities would be indistinguishable.
u/MisterYouAreSoDumb
The best way would be to NMR and Mass Spec it. Measuring trkB affinities is not really going to be a way to chemically validate a compound.
I already have a feeling the 7,8-DHF being sold on the market right now is a trihydroxyflavone, as our FTIR is seeing more hydroxyl groups. This is what started me down this path to begin with. Sigma Aldrich's reference standard has less hydroxyl groups than the THT, TLR, and supplier samples we have tested. So either Sigma Aldrich is wrong (highly doubtful), or people have been selling the complete wrong compound this whole time.