Comment · Thu, July 30, 2020 · ND Owner
⚠️ New Product Alert | Tongkat Ali Extract Powder | 2% Eurycomanone ⚠️
Original post in this thread
NootropicsDepotCom · 33 points
What they were answering
chemyd · 2 points
Coated tablets are definitely the way to go for this plant’s extracts. As someone who tried to understand the biological activity of this plant for a while, I’m curious why did you go with eurycomanone standardized material? I know people have focused on “eurypeptides” (not sure I believe that), saponins, alkaloids - and I couldn’t determine an advantage of any compound/class of compounds to focus on.
u/MisterYouAreSoDumb · ND Owner
Because eurycomanone is a really good marker compound that can be detected on a UV detector in an HPLC/UPLC or HP-TLC easily. Eurypeptides is a bullshit name that were made up and trademarked to fool people. There is no such thing as eurypeptides that a normal lab can verify. LJ100 used to be standardized to eurycomanone, but they removed that and started promoting the eurypeptide and glycosaponins thing. I have a bottle of it that used to say 1% eurycomanone, and you can find traces of them promoting it from 2017. Then they removed it and started promoting a made up name they tried to trademark... I have theories as to why they did that, but I won't get into those here to prevent legal issues. The main point is they used to standardize to eurycomanone at a higher level than is currently in there. Now it comes in at 0.5%. You can take a guess as to why they removed that from it. What would you do if you cut your standardization in half? I know, make up new names people can't verify or test in their lab!!!
Eurycomanone is THE marker compound for tongkat. It's not the only active, but it is the one best suited for QC standardization. If you increase eurycomanone, you increase the others. Also, look at the patent for LJ100.
https://patents.google.com/patent/US7132117B2/en?oq=7.132%2c117
They don't specify the actual peptides. They are a fucking mystery.
3. The composition of claim 1, wherein the peptide is a glycopeptide.
4. The composition of claim 1, wherein the peptide has about 30 –39 amino acids.
5. The composition of claim 4, wherein the peptide has about 36 amino acids.
Well slap my ass and call my Sally! Let me just go into the lab and test for random peptides in the 30-39 mer range! Ohh wait, that's fucking hard to do in a botanical sample. The methods for that are insanely difficult, and almost no labs in the US would be able to do it properly. To then set a standardization for that, and not tell anyone any details about what these peptides are? Pfft! Then to take off the main marker compound from the standardization? Come on now! Also, look at their fucking chromatogram!!!
Just garbage. I have poured through any research that I can find to try and identify these peptides. Supposedly the main one is around 4300 Daltons according to the patent. Jesus tittytwisting Christ! It's like CJC-1295! If fact, it's bigger than CJC-1295... That shit ain't making it through your GI tract intact! We are not injecting this tongkat ali extract... So what is that peptide doing, even if it is there?
So in the end, LJ100 used to be standardized to 1% eurycomanone. Now they completely removed it, and it has about 0.5%. They made up a new name for compounds that nobody can verify called eurypeptides, which even they admit are too big to absorb orally anyway. Also, anyone that knows peptides knows they are VERY difficult to properly test. They had to use SELDI, or surface-enhanced laser desorption/ionization, mass spectrometry. That is a complex soft ionization method that uses TOF, or time of flight, mass spectrometry. Good luck finding a lab that will do that for you. Also, there are massive issues with the validity and reproducibility of the results using SELDI mass spec. So much so that many scientists won't even use that methodology anymore. However, I can get validated reference standards for eurycomanone from multiple trustworthy suppliers, and I can actually build validated chemistry methods that are accurate and reproducible for its assay. It's the best suited marker compound in tongkat.